Hotspot

cemA-ycf4

LSC · 67146-68096 · 951 bp

Back to Gyrinops

Pi 0.00922

MarkerSeek score 55.60

Primer available yes

Label cemA-ycf4

Feature Metadata

label_name: cemA-ycf4
feature_type: igs
region: LSC
length_bp: 951
variable_sites: 25
pi: 0.00922
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA-ycf4_p1	GAGTGAAATCCACCAAGGCA	TATTTCTTGCGCGTACCGAT	50.0	45.0	58.1	57.8	1052-1065 bp	1.0	80.5
cemA-ycf4_p2	AGAGTGAAATCCACCAAGGC	TATTTCTTGCGCGTACCGAT	50.0	45.0	57.8	57.8	1053-1066 bp	1.0	79.6
cemA-ycf4_p3	GAGTGAAATCCACCAAGGCA	CGCGTACCGATTGAAGTCTT	50.0	50.0	58.1	58.4	1043-1056 bp	1.0	79.2
cemA-ycf4_p4	GAGTGAAATCCACCAAGGCA	CTATTTCTTGCGCGTACCGA	50.0	50.0	58.1	58.4	1053-1066 bp	1.0	78.6
cemA-ycf4_p5	AGAGTGAAATCCACCAAGGC	CGCGTACCGATTGAAGTCTT	50.0	50.0	57.8	58.4	1044-1057 bp	1.0	78.3