Hotspot

psbJ-petA

LSC · 64465-65241 · 777 bp

Back to Gyrinops

Pi 0.0115

MarkerSeek score 62.70

Primer available yes

Label psbJ-petA

Feature Metadata

label_name: psbJ-petA
feature_type: igs
region: LSC
length_bp: 777
variable_sites: 20
pi: 0.0115
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbJ-petA_p1	GCCGATACTACTGGAAGGAT	CATTACGTGTTCAGGGCCTT	50.0	50.0	56.2	58.2	1013-1021 bp	1.0	65.8
psbJ-petA_p2	GCCGATACTACTGGAAGGAT	GACCCATTACGTGTTCAGGG	50.0	55.0	56.2	58.3	1017-1025 bp	1.0	65.1
psbJ-petA_p3	GCCGATACTACTGGAAGGAT	TTACGTGTTCAGGGCCTTTT	50.0	45.0	56.2	57.6	1011-1019 bp	1.0	64.4
psbJ-petA_p4	GCCGATACTACTGGAAGGAT	TACGTGTTCAGGGCCTTTTG	50.0	50.0	56.2	58.4	1010-1018 bp	1.0	64.0
psbJ-petA_p5	GCCGATACTACTGGAAGGATT	CATTACGTGTTCAGGGCCTT	47.6	50.0	57.0	58.2	1013-1021 bp	1.0	63.9