Hotspot

psaA-ycf3

LSC · 44292-45266 · 975 bp

Back to Gyrinops

Pi 0.00804

MarkerSeek score 51.50

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 975
variable_sites: 17
pi: 0.00804
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TTCATGGGGAAGTGGGATCT	GGAAACAGGCTATAGCGCTT	50.0	50.0	58.0	58.0	1768-1776 bp	1.0	81.0
psaA-ycf3_p2	ACCTGAGCACTAGGTCCAAT	GGAAACAGGCTATAGCGCTT	50.0	50.0	58.0	58.0	1392-1400 bp	1.0	81.0
psaA-ycf3_p3	TTCATGGGGAAGTGGGATCT	AATTGACCGCCTATTCCGAC	50.0	50.0	58.0	58.1	1861-1869 bp	1.0	80.9
psaA-ycf3_p4	AAAGAGACCCAAGCCCTAGT	GGAAACAGGCTATAGCGCTT	50.0	50.0	58.0	58.0	1676-1684 bp	1.0	80.8
psaA-ycf3_p5	TTCATGGGGAAGTGGGATCT	GAGCCGATCCCCTAAGGTAT	50.0	55.0	58.0	58.1	2272-2280 bp	1.0	80.7