Hotspot

rps2-rpoC2

LSC · 17647-17974 · 328 bp

Back to Gyrinops

Pi 0.00711

MarkerSeek score 61.10

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 328
variable_sites: 6
pi: 0.00711
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	GATATAGGGCGCCATTCGAG	CCCTCTTCAAGGCAGCATAA	55.0	50.0	58.1	57.6	549-566 bp	1.0	78.0
rps2-rpoC2_p2	GCTTCCAGCATCTCTTCCAA	CCCTCTTCAAGGCAGCATAA	50.0	50.0	57.9	57.6	491-508 bp	1.0	77.9
rps2-rpoC2_p3	AATGAACTCCTGCTTCCAGC	CCCTCTTCAAGGCAGCATAA	50.0	50.0	58.2	57.6	502-519 bp	1.0	77.5
rps2-rpoC2_p4	CGCCATTCGAGGATTCCATT	CCCTCTTCAAGGCAGCATAA	50.0	50.0	58.4	57.6	540-557 bp	1.0	75.4
rps2-rpoC2_p5	TGCATAGATATAGGGCGCCA	CCCTCTTCAAGGCAGCATAA	50.0	50.0	58.4	57.6	555-572 bp	1.0	75.1