Hotspot

atpH-atpI

LSC · 14720-15938 · 1219 bp

Back to Gyrinops

Pi 0.00609

MarkerSeek score 58.20

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1219
variable_sites: 20
pi: 0.00609
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.0	57.9	1306-1308 bp	1.0	79.9
atpH-atpI_p2	AGCGGCAGAAATCAATGGAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	57.9	57.9	1298-1300 bp	1.0	79.7
atpH-atpI_p3	GAAGCAAGTCCGACAGCTAA	TTTTGCAACTTTAGCTGCGG	50.0	45.0	57.9	57.9	1336-1338 bp	1.0	79.5
atpH-atpI_p4	AAGCGGCAGAAATCAATGGA	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.2	57.9	1299-1301 bp	1.0	78.9
atpH-atpI_p5	AATAACGGAAGCGGCAGAAA	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.2	57.9	1307-1309 bp	1.0	78.7