Hotspot

rpl16

LSC · 80540-81927 · 1388 bp

Back to Gypsophila

Pi 0.00288
MarkerSeek score 55.00
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1388
variable_sites
8
pi
0.00288
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 TCTGTTCTTTGAATGCAAAAA ACCTAGTTCTAATTTTTAATTCCACA 28.6 26.9 52.7 55.1 1553-1567 bp 1.0 40.9
rpl16_p2 TTCTGTTCTTTGAATGCAAAA ACCTAGTTCTAATTTTTAATTCCACA 28.6 26.9 52.7 55.1 1554-1568 bp 1.0 40.9
rpl16_p3 TTTCTGTTCTTTGAATGCAAA ACCTAGTTCTAATTTTTAATTCCACA 28.6 26.9 52.7 55.1 1555-1569 bp 1.0 40.9
rpl16_p4 TTTTCTGTTCTTTGAATGCAA ACCTAGTTCTAATTTTTAATTCCACA 28.6 26.9 52.7 55.1 1556-1570 bp 1.0 40.9
rpl16_p5 TTTTTCTGTTCTTTGAATGCA ACCTAGTTCTAATTTTTAATTCCACA 28.6 26.9 52.7 55.1 1557-1571 bp 1.0 40.9