Hotspot

ndhD

SSC · 114630-116132 · 1503 bp

Back to Gypsophila

Pi 0.00266

MarkerSeek score 45.60

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 8
pi: 0.00266
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	CACAAACATCAAGCATGCCC	TTTTGGCCCAAGTGTATCTT	50.0	40.0	58.3	55.1	1616 bp	1.0	55.1
ndhD_p2	CACAAACATCAAGCATGCCC	TTTTTGGCCCAAGTGTATCT	50.0	40.0	58.3	55.1	1617 bp	1.0	55.1
ndhD_p3	CACAAACATCAAGCATGCCC	TTTTTGGCCCAAGTGTATCTT	50.0	38.1	58.3	55.9	1617 bp	1.0	53.7
ndhD_p4	CAAACATCAAGCATGCCCAA	TTTTGGCCCAAGTGTATCTT	45.0	40.0	57.5	55.1	1614 bp	1.0	53.7
ndhD_p5	CAAACATCAAGCATGCCCAA	TTTTTGGCCCAAGTGTATCT	45.0	40.0	57.5	55.1	1615 bp	1.0	53.7