Hotspot

clpP1

LSC · 69192-71314 · 2123 bp

Back to Gypsophila

Pi 0.00141

MarkerSeek score 59.40

Primer available yes

Label clpP1

Feature Metadata

label_name: clpP1
feature_type: gene
region: LSC
length_bp: 2123
variable_sites: 5
pi: 0.00141
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP1_p1	AGATCAATCCTAACCGAATGCA	ACGTTTTCACATCAAAGTGA	40.9	35.0	57.6	53.7	2248-2300 bp	1.0	41.0
clpP1_p2	AGATCAATCCTAACCGAATGCA	TCACATCAAAGTGAACCATAGT	40.9	36.4	57.6	55.4	2242-2294 bp	1.0	41.0
clpP1_p3	GATCAATCCTAACCGAATGCA	ACGTTTTCACATCAAAGTGA	42.9	35.0	56.1	53.7	2247-2299 bp	1.0	41.0
clpP1_p4	AGATCAATCCTAACCGAATGCAT	ACGTTTTCACATCAAAGTGA	39.1	35.0	58.0	53.7	2248-2300 bp	1.0	41.0
clpP1_p5	AGATCAATCCTAACCGAATGCA	TCACATCAAAGTGAACCATAGTACT	40.9	36.0	57.6	58.0	2242-2294 bp	1.0	41.0