Hotspot

trnS-GCU-trnG-UCC

LSC · 7940-8757 · 818 bp

Back to Gypsophila

Pi 0.00637
MarkerSeek score 65.30
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
818
variable_sites
10
pi
0.00637
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CACGGAAAGAGAGGGATTCG TCAAACCGGTAGACCCCTTA 55.0 50.0 58.1 57.7 983-1117 bp 1.0 78.8
trnS-GCU-trnG-UCC_p2 CACGGAAAGAGAGGGATTCG AACCGGTAGACCCCTTAACT 55.0 50.0 58.1 57.7 980-1114 bp 1.0 78.8
trnS-GCU-trnG-UCC_p3 CACGGAAAGAGAGGGATTCG AATCAAACCGGTAGACCCCT 55.0 50.0 58.1 58.3 985-1119 bp 1.0 78.7
trnS-GCU-trnG-UCC_p4 CACGGAAAGAGAGGGATTCG ATCAAACCGGTAGACCCCTT 55.0 50.0 58.1 58.3 984-1118 bp 1.0 78.7
trnS-GCU-trnG-UCC_p5 ACGGAAAGAGAGGGATTCGA TCAAACCGGTAGACCCCTTA 50.0 50.0 58.2 57.7 982-1116 bp 1.0 78.2