Hotspot

ycf1

SSC · 107391-112442 · 5052 bp

Back to Gynura

Pi 0.00139
MarkerSeek score 50.10
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5052
variable_sites
7
pi
0.00139
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 TTCAAGTTGAACCTAATAAGGACA AAGTTGCTTGGGATAAACCA 33.3 40.0 56.0 55.1 256 bp 1.0 40.6
ycf1_p2 TTCAAGTTGAACCTAATAAGGACA AGTAAGTTGCTTGGGATAAACCA 33.3 39.1 56.0 58.0 259 bp 1.0 40.6
ycf1_p3 TTCAAGTTGAACCTAATAAGGACA TGGGATAAACCAAGGTTTCTCT 33.3 40.9 56.0 57.0 248 bp 1.0 40.6
ycf1_p4 TTCAAGTTGAACCTAATAAGGACAA AAGTTGCTTGGGATAAACCA 32.0 40.0 56.6 55.1 256 bp 1.0 40.6
ycf1_p5 TTCAAGTTGAACCTAATAAGGACA TGGGATAAACCAAGGTTTCTCTT 33.3 39.1 56.0 57.7 248 bp 1.0 40.6