Hotspot

trnL(TAG)-rpl32

SSC · 121900-122705 · 806 bp

Back to Gynura

Pi 0.00498
MarkerSeek score 62.00
Primer available yes
Label trnL(TAG)-rpl32

Feature Metadata

label_name
trnL(TAG)-rpl32
feature_type
igs
region
SSC
length_bp
806
variable_sites
4
pi
0.00498
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL(TAG)-rpl32_p1 AGCGTGTCTACCGATTTCAC GGGAAATCTCTTTCTACCGGG 50.0 52.4 58.0 57.8 895-898 bp 1.0 71.0
trnL(TAG)-rpl32_p2 CAGCGTGTCTACCGATTTCA GGGAAATCTCTTTCTACCGGG 50.0 52.4 58.0 57.8 896-899 bp 1.0 71.0
trnL(TAG)-rpl32_p3 TCCTAAGAGCAGCGTGTCTA GGGAAATCTCTTTCTACCGGG 50.0 52.4 57.9 57.8 905-908 bp 1.0 70.1
trnL(TAG)-rpl32_p4 CCTAAGAGCAGCGTGTCTAC GGGAAATCTCTTTCTACCGGG 55.0 52.4 57.8 57.8 904-907 bp 1.0 69.5
trnL(TAG)-rpl32_p5 CTAAGAGCAGCGTGTCTACC GGGAAATCTCTTTCTACCGGG 55.0 52.4 57.8 57.8 903-906 bp 1.0 69.5