Hotspot

ndhI-ndhG

SSC · 116952-117303 · 352 bp

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Pi 0.0114
MarkerSeek score 60.10
Primer available yes
Label ndhI-ndhG

Feature Metadata

label_name
ndhI-ndhG
feature_type
igs
region
SSC
length_bp
352
variable_sites
4
pi
0.0114
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhI-ndhG_p1 TCAAATTGCTTTGGGTCGTT AGACCCAATCCCAGAAAGAC 40.0 50.0 56.4 56.8 496 bp 1.0 58.7
ndhI-ndhG_p2 TCAAATTGCTTTGGGTCGTT GACCCAATCCCAGAAAGACT 40.0 50.0 56.4 56.8 495 bp 1.0 58.7
ndhI-ndhG_p3 TCAAATTGCTTTGGGTCGTT AGACCCAATCCCAGAAAGACT 40.0 47.6 56.4 58.4 496 bp 1.0 57.3
ndhI-ndhG_p4 ATCAAATTGCTTTGGGTCGT AGACCCAATCCCAGAAAGAC 40.0 50.0 56.2 56.8 497 bp 1.0 56.9
ndhI-ndhG_p5 ATCAAATTGCTTTGGGTCGT GACCCAATCCCAGAAAGACT 40.0 50.0 56.2 56.8 496 bp 1.0 56.9