Hotspot

atpB-rbcL

LSC · 54016-54749 · 734 bp

Back to Gynura

Pi 0.00273
MarkerSeek score 60.60
Primer available yes
Label atpB-rbcL

Feature Metadata

label_name
atpB-rbcL
feature_type
igs
region
LSC
length_bp
734
variable_sites
2
pi
0.00273
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB-rbcL_p1 ACGCCCTAGGTTTGTTTTGT GCTTTGAATCCAACACTCGC 45.0 50.0 57.9 58.0 842-844 bp 1.0 79.6
atpB-rbcL_p2 GTTTTGTCAAGCGTGGGAAC GCTTTGAATCCAACACTCGC 50.0 50.0 58.2 58.0 829-831 bp 1.0 78.8
atpB-rbcL_p3 TTTGCCTGGCGGAAAAGATA GCTTTGAATCCAACACTCGC 45.0 50.0 57.8 58.0 893-895 bp 1.0 78.4
atpB-rbcL_p4 TTTGTTTTGTCAAGCGTGGG GCTTTGAATCCAACACTCGC 45.0 50.0 57.7 58.0 832-834 bp 1.0 77.9
atpB-rbcL_p5 CGATACGCCCTAGGTTTGTT GCTTTGAATCCAACACTCGC 50.0 50.0 57.7 58.0 846-848 bp 1.0 77.7