Hotspot

trnT(TGT)-trnL(TAA)

LSC · 46388-46949 · 562 bp

Back to Gynura

Pi 0.00356
MarkerSeek score 60.00
Primer available yes
Label trnT(TGT)-trnL(TAA)

Feature Metadata

label_name
trnT(TGT)-trnL(TAA)
feature_type
igs
region
LSC
length_bp
562
variable_sites
2
pi
0.00356
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT(TGT)-trnL(TAA)_p1 GCGATGCTCTAACCTCTGAG CCGTAGCGTCTACCGATTTC 55.0 55.0 57.9 58.2 623 bp 1.0 77.2
trnT(TGT)-trnL(TAA)_p2 GGAATCGAACCGATGACCAT CCGTAGCGTCTACCGATTTC 50.0 55.0 57.8 58.2 655 bp 1.0 76.3
trnT(TGT)-trnL(TAA)_p3 GCGATGCTCTAACCTCTGAG CGTCTACCGATTTCGCCATA 55.0 50.0 57.9 57.6 617 bp 1.0 75.9
trnT(TGT)-trnL(TAA)_p4 GGAATCGAACCGATGACCAT CGTCTACCGATTTCGCCATA 50.0 50.0 57.8 57.6 649 bp 1.0 75.0
trnT(TGT)-trnL(TAA)_p5 GCGATGCTCTAACCTCTGAG CGTAGCGTCTACCGATTTCG 55.0 55.0 57.9 58.6 622 bp 1.0 74.3