Hotspot

trnS(GCT)-psbZ

LSC · 35148-35489 · 342 bp

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Pi 0.00292
MarkerSeek score 55.10
Primer available yes
Label trnS(GCT)-psbZ

Feature Metadata

label_name
trnS(GCT)-psbZ
feature_type
igs
region
LSC
length_bp
342
variable_sites
1
pi
0.00292
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS(GCT)-psbZ_p1 CAACCACTCAGCCATCTCTC CCAACCATCTGGAGAAGCAA 55.0 50.0 58.0 57.8 462 bp 1.0 78.4
trnS(GCT)-psbZ_p2 CAACCACTCAGCCATCTCTC GACCAACCATCTGGAGAAGC 55.0 55.0 58.0 58.3 464 bp 1.0 78.0
trnS(GCT)-psbZ_p3 TCAACCACTCAGCCATCTCT CCAACCATCTGGAGAAGCAA 50.0 50.0 58.4 57.8 463 bp 1.0 75.7
trnS(GCT)-psbZ_p4 TCAACCACTCAGCCATCTCT GACCAACCATCTGGAGAAGC 50.0 55.0 58.4 58.3 465 bp 1.0 75.3
trnS(GCT)-psbZ_p5 TTCAACCACTCAGCCATCTC CCAACCATCTGGAGAAGCAA 50.0 50.0 57.5 57.8 464 bp 1.0 74.7