Hotspot

trnE(TTC)-rpoB

LSC · 11972-12959 · 988 bp

Back to Gynura

Pi 0.00405
MarkerSeek score 57.50
Primer available yes
Label trnE(TTC)-rpoB

Feature Metadata

label_name
trnE(TTC)-rpoB
feature_type
igs
region
LSC
length_bp
988
variable_sites
4
pi
0.00405
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE(TTC)-rpoB_p1 GGGACTTGAACCCTCGTTTT GTTCTTCTGTCAAACCCCGA 50.0 50.0 58.0 57.8 1129-1165 bp 1.0 78.1
trnE(TTC)-rpoB_p2 GGGACTTGAACCCTCGTTTT AGTTCTTCTGTCAAACCCCG 50.0 50.0 58.0 57.7 1130-1166 bp 1.0 78.0
trnE(TTC)-rpoB_p3 TAACCACTGGACCATAGGGG GTTCTTCTGTCAAACCCCGA 55.0 50.0 58.1 57.8 1088-1124 bp 1.0 77.4
trnE(TTC)-rpoB_p4 TAACCACTGGACCATAGGGG AGTTCTTCTGTCAAACCCCG 55.0 50.0 58.1 57.7 1089-1125 bp 1.0 77.3
trnE(TTC)-rpoB_p5 CCCTCGTTTTCTCCGTGAAA GTTCTTCTGTCAAACCCCGA 50.0 50.0 58.1 57.8 1119-1155 bp 1.0 77.3