Hotspot

trnD(GTC)-trnY(GTA)

LSC · 11543-11624 · 82 bp

Back to Gynura

Pi 0.0122
MarkerSeek score 59.50
Primer available yes
Label trnD(GTC)-trnY(GTA)

Feature Metadata

label_name
trnD(GTC)-trnY(GTA)
feature_type
igs
region
LSC
length_bp
82
variable_sites
1
pi
0.0122
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD(GTC)-trnY(GTA)_p1 CGGTGCTCTAGCCTATTGAA GAATTAGGCGTTGGGTCGAT 50.0 50.0 57.1 58.1 208 bp 1.0 72.9
trnD(GTC)-trnY(GTA)_p2 GTACTTATTGGATCCGTCGGG GAATTAGGCGTTGGGTCGAT 52.4 50.0 58.0 58.1 269 bp 1.0 71.7
trnD(GTC)-trnY(GTA)_p3 GCGGTGCTCTAGCCTATTG GAATTAGGCGTTGGGTCGAT 57.9 50.0 58.1 58.1 209 bp 1.0 71.2
trnD(GTC)-trnY(GTA)_p4 CGGTGCTCTAGCCTATTGAA AATTAGGCGTTGGGTCGATG 50.0 50.0 57.1 58.3 207 bp 1.0 70.6
trnD(GTC)-trnY(GTA)_p5 CGGTGCTCTAGCCTATTGAA ATGAATTAGGCGTTGGGTCG 50.0 50.0 57.1 58.3 210 bp 1.0 70.6