Hotspot

matK-trnK(TTT)

LSC · 3436-4185 · 750 bp

Back to Gynura

Pi 0.00134
MarkerSeek score 59.60
Primer available yes
Label matK-trnK(TTT)

Feature Metadata

label_name
matK-trnK(TTT)
feature_type
igs
region
LSC
length_bp
750
variable_sites
1
pi
0.00134
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-trnK(TTT)_p1 ACCATGATCATGAGCAAGTACA AACGGTAGAGTACTCGGCTT 40.9 50.0 57.5 58.2 881-882 bp 1.0 58.9
matK-trnK(TTT)_p2 ACCATGATCATGAGCAAGTACA ACGGTAGAGTACTCGGCTTT 40.9 50.0 57.5 58.2 880-881 bp 1.0 58.9
matK-trnK(TTT)_p3 ACCATGATCATGAGCAAGTACA TTGGGTTGCTAACTCAACGG 40.9 50.0 57.5 58.4 896-897 bp 1.0 57.0
matK-trnK(TTT)_p4 ACCATGATCATGAGCAAGTACA GGGTTGCTAACTCAACGGTA 40.9 50.0 57.5 57.5 894-895 bp 1.0 56.6
matK-trnK(TTT)_p5 AACCATGATCATGAGCAAGT ACGGTAGAGTACTCGGCTTT 40.0 50.0 55.0 58.2 881-882 bp 1.0 54.9