Hotspot

rps3

LSC · 81740-82396 · 657 bp

Back to Gynoxys

Pi 0.00266

MarkerSeek score 37.60

Primer available yes

Label rps3

Feature Metadata

label_name: rps3
feature_type: gene
region: LSC
length_bp: 657
variable_sites: 5
pi: 0.00266
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3_p1	GTTCGATGAGCAGATCCGTT	GAGGGCGTAGTTTTGCGATA	50.0	50.0	58.1	58.1	1354-1356 bp	1.0	79.6
rps3_p2	TTGTTCGATGAGCAGATCCG	GAGGGCGTAGTTTTGCGATA	50.0	50.0	58.1	58.1	1356-1358 bp	1.0	79.6
rps3_p3	CCGTTTAATGAGGAACCCGT	GAGGGCGTAGTTTTGCGATA	50.0	50.0	57.9	58.1	1339-1341 bp	1.0	79.3
rps3_p4	GTTCGATGAGCAGATCCGTT	TCACAAAGCGCGAAGAGTAA	50.0	45.0	58.1	57.9	1581-1583 bp	1.0	79.0
rps3_p5	TTGTTCGATGAGCAGATCCG	TCACAAAGCGCGAAGAGTAA	50.0	45.0	58.1	57.9	1583-1585 bp	1.0	79.0