Hotspot

clpP

LSC · 68929-70943 · 2015 bp

Back to Gynoxys

Pi 0.00105

MarkerSeek score 58.80

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2015
variable_sites: 7
pi: 0.00105
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	ATTGACGCGAAATACAGGCT	GGCTTTGTTACGTTTCCACA	45.0	45.0	58.0	56.9	2101-2106 bp	1.0	71.1
clpP_p2	CTCGGCAGGGTTAACTTCAA	GGCTTTGTTACGTTTCCACA	50.0	45.0	57.8	56.9	2128-2133 bp	1.0	69.8
clpP_p3	AACTCGGCAGGGTTAACTTC	GGCTTTGTTACGTTTCCACA	50.0	45.0	57.8	56.9	2130-2135 bp	1.0	69.8
clpP_p4	ATTGACGCGAAATACAGGCT	AGGCTTTGTTACGTTTCCACA	45.0	42.9	58.0	58.4	2102-2107 bp	1.0	69.4
clpP_p5	ATTGACGCGAAATACAGGCT	AGGCTTTGTTACGTTTCCAC	45.0	45.0	58.0	56.6	2102-2107 bp	1.0	68.7