Hotspot

rpl32-ndhF

SSC · 122861-123927 · 1067 bp

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Pi 0.00594

MarkerSeek score 53.80

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 1067
variable_sites: 16
pi: 0.00594
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	ACAAAGCTTTTAACGCTGCC	GTTGCCGTCGGAAAAAGAAG	45.0	50.0	57.9	58.0	1261-1269 bp	1.0	79.8
rpl32-ndhF_p2	AACGCTGCCCAATATCCTTT	GTTGCCGTCGGAAAAAGAAG	45.0	50.0	57.9	58.0	1250-1258 bp	1.0	79.7
rpl32-ndhF_p3	ACAAAGCTTTTAACGCTGCC	TGCCGTCGGAAAAAGAAGAA	45.0	45.0	57.9	58.0	1259-1267 bp	1.0	79.7
rpl32-ndhF_p4	ACAAAGCTTTTAACGCTGCC	TTGCCGTCGGAAAAAGAAGA	45.0	45.0	57.9	58.0	1260-1268 bp	1.0	79.7
rpl32-ndhF_p5	ACAAAGCTTTTAACGCTGCC	TACTTTTTGTTGCCGTCGGA	45.0	45.0	57.9	58.0	1269-1277 bp	1.0	79.7