Hotspot

trnL-TAG-rpl32

SSC · 121884-122695 · 812 bp

Back to Gynoxys

Pi 0.00623
MarkerSeek score 63.80
Primer available yes
Label trnL-TAG-rpl32

Feature Metadata

label_name
trnL-TAG-rpl32
feature_type
igs
region
SSC
length_bp
812
variable_sites
12
pi
0.00623
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-TAG-rpl32_p1 AGCGTGTCTACCGATTTCAC GGCAGCGTTAAAAGCTTTGT 50.0 45.0 58.0 57.9 909-933 bp 1.0 82.4
trnL-TAG-rpl32_p2 CAGCGTGTCTACCGATTTCA GGCAGCGTTAAAAGCTTTGT 50.0 45.0 58.0 57.9 910-934 bp 1.0 82.4
trnL-TAG-rpl32_p3 AGCGTGTCTACCGATTTCAC AAAGGATATTGGGCAGCGTT 50.0 45.0 58.0 57.9 920-944 bp 1.0 82.3
trnL-TAG-rpl32_p4 CAGCGTGTCTACCGATTTCA AAAGGATATTGGGCAGCGTT 50.0 45.0 58.0 57.9 921-945 bp 1.0 82.3
trnL-TAG-rpl32_p5 TCCTAAGAGCAGCGTGTCTA GGCAGCGTTAAAAGCTTTGT 50.0 45.0 57.9 57.9 919-943 bp 1.0 81.4