Hotspot

psaA-ycf3

LSC · 41199-41888 · 690 bp

Back to Gynoxys

Pi 0.0076

MarkerSeek score 46.50

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 690
variable_sites: 12
pi: 0.0076
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCATCAGCATGTAGGTTCCA	TTCTGAAATTGCGGAGGCTT	50.0	45.0	58.0	58.1	967-973 bp	1.0	80.7
psaA-ycf3_p2	CACTAGGCCCAATGTGAGTC	TTCTGAAATTGCGGAGGCTT	55.0	45.0	58.0	58.1	1143-1149 bp	1.0	80.6
psaA-ycf3_p3	GTTATTCCAGATGCTCGCCA	TTCTGAAATTGCGGAGGCTT	50.0	45.0	58.0	58.1	1261-1267 bp	1.0	80.3
psaA-ycf3_p4	ATTCCAGATGCTCGCCAAAT	TTCTGAAATTGCGGAGGCTT	45.0	45.0	57.9	58.1	1258-1264 bp	1.0	80.2
psaA-ycf3_p5	CCCCGCTAAATGGTGATTCA	TTCTGAAATTGCGGAGGCTT	50.0	45.0	57.9	58.1	1412-1418 bp	1.0	80.1