Hotspot

psbC-trnS-TGA

LSC · 34654-34885 · 232 bp

Back to Gynoxys

Pi 0.00866
MarkerSeek score 60.40
Primer available yes
Label psbC-trnS-TGA

Feature Metadata

label_name
psbC-trnS-TGA
feature_type
igs
region
LSC
length_bp
232
variable_sites
5
pi
0.00866
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbC-trnS-TGA_p1 TCTTCGTAGGGCATTTGTGG GAGAGATGGCTGAGTGGTTG 50.0 55.0 57.9 58.0 424-427 bp 1.0 80.5
psbC-trnS-TGA_p2 CCTGTTCTTTCCATGACCCC GAGAGATGGCTGAGTGGTTG 55.0 55.0 58.2 58.0 341-344 bp 1.0 80.4
psbC-trnS-TGA_p3 TCTTCGTAGGGCATTTGTGG GTCTTTCGGAGAGATGGCTG 50.0 55.0 57.9 58.1 432-435 bp 1.0 80.1
psbC-trnS-TGA_p4 CCTGTTCTTTCCATGACCCC GTCTTTCGGAGAGATGGCTG 55.0 55.0 58.2 58.1 349-352 bp 1.0 80.0
psbC-trnS-TGA_p5 TCTTCGTAGGGCATTTGTGG TCTTTCGGAGAGATGGCTGA 50.0 50.0 57.9 58.1 431-434 bp 1.0 79.4