Hotspot

trnE-TTC-rpoB

LSC · 12192-12941 · 750 bp

Back to Gynoxys

Pi 0.0019

MarkerSeek score 54.40

Primer available yes

Label trnE-TTC-rpoB

Feature Metadata

label_name: trnE-TTC-rpoB
feature_type: igs
region: LSC
length_bp: 750
variable_sites: 5
pi: 0.0019
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-TTC-rpoB_p1	GGGAGTTGAACCCTCGTTTT	GTTCTTCTGTCAAACCCCGA	50.0	50.0	58.0	57.8	884-893 bp	1.0	78.9
trnE-TTC-rpoB_p2	GGGAGTTGAACCCTCGTTTT	AGTTCTTCTGTCAAACCCCG	50.0	50.0	58.0	57.7	885-894 bp	1.0	78.8
trnE-TTC-rpoB_p3	TAACCACTGGACCATAGGGG	GTTCTTCTGTCAAACCCCGA	55.0	50.0	58.1	57.8	843-852 bp	1.0	78.2
trnE-TTC-rpoB_p4	TAACCACTGGACCATAGGGG	AGTTCTTCTGTCAAACCCCG	55.0	50.0	58.1	57.7	844-853 bp	1.0	78.2
trnE-TTC-rpoB_p5	CCCTCGTTTTCTCCGTGAAA	GTTCTTCTGTCAAACCCCGA	50.0	50.0	58.1	57.8	874-883 bp	1.0	78.1