Hotspot

trnS-GCT-trnC-GCA

LSC · 8704-9447 · 744 bp

Back to Gynoxys

Pi 0.0049

MarkerSeek score 50.60

Primer available yes

Label trnS-GCT-trnC-GCA

Feature Metadata

label_name: trnS-GCT-trnC-GCA
feature_type: igs
region: LSC
length_bp: 744
variable_sites: 8
pi: 0.0049
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCT-trnC-GCA_p1	ACGGAAAGAGAGGGATTCGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.2	58.1	895 bp	1.0	78.8
trnS-GCT-trnC-GCA_p2	ACGGAAAGAGAGGGATTCGA	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.2	58.4	877 bp	1.0	76.7
trnS-GCT-trnC-GCA_p3	CTTTCGTCCACTCAGCCATC	CCGGATTTGAACTGGGGAAA	55.0	50.0	58.6	58.1	831 bp	1.0	75.0
trnS-GCT-trnC-GCA_p4	AACGGAAAGAGAGGGATTCG	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.3	58.1	896 bp	1.0	74.7
trnS-GCT-trnC-GCA_p5	CTTTCGTCCACTCAGCCATC	AAAAGGATTTGCAGTCCCCC	55.0	50.0	58.6	58.4	813 bp	1.0	72.9