Hotspot

rps16-trnQ-TTG

LSC · 6349-7318 · 970 bp

Back to Gynoxys

Pi 0.00502

MarkerSeek score 52.40

Primer available yes

Label rps16-trnQ-TTG

Feature Metadata

label_name: rps16-trnQ-TTG
feature_type: igs
region: LSC
length_bp: 970
variable_sites: 11
pi: 0.00502
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-TTG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1030-1051 bp	1.0	80.1
rps16-trnQ-TTG_p2	AGAATAAGAATCCACGCCGG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.8	58.1	1062-1083 bp	1.0	79.3
rps16-trnQ-TTG_p3	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	1044-1065 bp	1.0	78.3
rps16-trnQ-TTG_p4	CGGATCGTGTCCTTCAAGTC	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	1045-1066 bp	1.0	78.2
rps16-trnQ-TTG_p5	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	45.0	50.0	58.1	57.3	1034-1055 bp	1.0	75.4