Hotspot

rpl32-trnL-UAG

SSC · 115966-117272 · 1307 bp

Back to Gynostemma

Pi 0.0297

MarkerSeek score 75.50

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1307
variable_sites: 140
pi: 0.0297
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGCAGCGTTGAAAGCTTTTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.4	58.0	1343-1477 bp	1.0	88.5
rpl32-trnL-UAG_p2	GGCAGCGTTGAAAGCTTTTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	58.4	58.0	1344-1478 bp	1.0	88.5
rpl32-trnL-UAG_p3	GGCAGCGTTGAAAGCTTTTT	GGAGAGCGGGGACTAATAGT	45.0	55.0	58.4	58.0	1923-2057 bp	1.0	87.4
rpl32-trnL-UAG_p4	GGCAGCGTTGAAAGCTTTTT	TGGAGAGCGGGGACTAATAG	45.0	55.0	58.4	58.0	1924-2058 bp	1.0	87.4
rpl32-trnL-UAG_p5	GGCAGCGTTGAAAGCTTTTT	CGGGGACTAATAGTGGGGAT	45.0	55.0	58.4	58.0	1917-2051 bp	0.833333	78.9