Hotspot

ndhF-rpl32

SSC · 115197-115803 · 607 bp

Back to Gynostemma

Pi 0.0242

MarkerSeek score 71.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 607
variable_sites: 49
pi: 0.0242
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGTTGCCGTCGGAAAAAGTA	TCCAAATATTTTTCCGAATCCGT	45.0	34.8	58.0	56.8	762-832 bp	1.0	52.4
ndhF-rpl32_p2	TTTGTTGCCGTCGGAAAAAG	TCCAAATATTTTTCCGAATCCGT	45.0	34.8	57.8	56.8	764-834 bp	1.0	51.2
ndhF-rpl32_p3	TGTTGCCGTCGGAAAAAGTA	TCCAAATATTTTTCCGAATCCGTT	45.0	33.3	58.0	57.5	762-832 bp	1.0	49.6
ndhF-rpl32_p4	TGTTGCCGTCGGAAAAAGTA	TTCCAAATATTTTTCCGAATCCGT	45.0	33.3	58.0	57.5	763-833 bp	1.0	49.5
ndhF-rpl32_p5	TGTTGCCGTCGGAAAAAGTA	CCAAATATTTTTCCGAATCCGT	45.0	36.4	58.0	55.4	761-831 bp	1.0	49.0