Hotspot

trnL-UAA-trnF-GAA

LSC · 50229-50580 · 352 bp

Back to Gynostemma

Pi 0.022

MarkerSeek score 70.10

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 352
variable_sites: 23
pi: 0.022
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	439-482 bp	1.0	82.4
trnL-UAA-trnF-GAA_p2	AATCGTGAGGGTTCAAGTCC	GTGCCAGGAACCAGATTTGA	50.0	50.0	57.5	58.1	454-497 bp	1.0	81.9
trnL-UAA-trnF-GAA_p3	AATCGTGAGGGTTCAAGTCC	CATGTGCCAGGAACCAGATT	50.0	50.0	57.5	58.2	457-500 bp	1.0	81.3
trnL-UAA-trnF-GAA_p4	AATCGTGAGGGTTCAAGTCC	TTCATCATGTGCCAGGAACC	50.0	50.0	57.5	58.2	462-505 bp	1.0	81.2
trnL-UAA-trnF-GAA_p5	AATCGTGAGGGTTCAAGTCC	TGTGCCAGGAACCAGATTTG	50.0	50.0	57.5	58.4	455-498 bp	1.0	79.5