Hotspot

trnT-UGU-trnL-UAA

LSC · 48582-49591 · 1010 bp

Back to Gynostemma

Pi 0.0209

MarkerSeek score 71.20

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 1010
variable_sites: 65
pi: 0.0209
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	55.0	50.0	57.9	57.6	1046-1187 bp	1.0	84.5
trnT-UGU-trnL-UAA_p2	GCGATGCTCTAACCTCTGAG	GGTTTCCATACCAAGGCTCA	55.0	50.0	57.9	57.5	1084-1225 bp	1.0	83.6
trnT-UGU-trnL-UAA_p3	GGAATCGAACCGATGACCAT	CGTCTACCGATTTCGCCATA	50.0	50.0	57.8	57.6	1078-1219 bp	1.0	83.5
trnT-UGU-trnL-UAA_p4	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	1052-1193 bp	0.916667	81.6
trnT-UGU-trnL-UAA_p5	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.8	58.2	1084-1225 bp	0.916667	80.7