Hotspot

trnT-GGU-psbD

LSC · 33216-34778 · 1563 bp

Back to Gynostemma

Pi 0.0122

MarkerSeek score 62.00

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1563
variable_sites: 61
pi: 0.0122
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GGCGTAAGTCATCGGTTCAA	TCCCTCCGTAACCAGTCATC	50.0	55.0	58.3	58.5	1658-1702 bp	1.0	79.8
trnT-GGU-psbD_p2	GGCGTAAGTCATCGGTTCAA	GTCCCTCCGTAACCAGTCAT	50.0	55.0	58.3	58.8	1659-1703 bp	1.0	77.4
trnT-GGU-psbD_p3	GGCGTAAGTCATCGGTTCAA	CCTCCGTAACCAGTCATCCA	50.0	55.0	58.3	58.8	1656-1700 bp	1.0	77.4
trnT-GGU-psbD_p4	GGCGTAAGTCATCGGTTCAA	CTCCGTAACCAGTCATCCAT	50.0	50.0	58.3	56.7	1655-1699 bp	1.0	73.7
trnT-GGU-psbD_p5	GTTCAAATCCGATAAGGGGCT	TCCCTCCGTAACCAGTCATC	47.6	55.0	58.1	58.5	1644-1688 bp	1.0	73.5