Hotspot

atpA-atpF

LSC · 12103-12164 · 62 bp

Back to Gynostemma

Pi 0.0412

MarkerSeek score 69.60

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 62
variable_sites: 6
pi: 0.0412
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	ACTTGAAGTACGGTACCGGT	ATTGGCATGTTTGGGACGAT	50.0	45.0	58.4	58.1	204-209 bp	1.0	80.4
atpA-atpF_p2	TTGCTCAATACGTTCACGGA	ATTGGCATGTTTGGGACGAT	45.0	45.0	57.6	58.1	157-162 bp	1.0	80.3
atpA-atpF_p3	TGCTCAATACGTTCACGGAT	ATTGGCATGTTTGGGACGAT	45.0	45.0	57.3	58.1	156-161 bp	1.0	78.5
atpA-atpF_p4	ACTTGAAGTACGGTACCGGT	TATTGGCATGTTTGGGACGA	50.0	45.0	58.4	57.5	205-210 bp	1.0	77.6
atpA-atpF_p5	TTGCTCAATACGTTCACGGA	TATTGGCATGTTTGGGACGA	45.0	45.0	57.6	57.5	158-163 bp	1.0	77.5