Hotspot

trnk-UUU-rps16

LSC · 4399-5520 · 1122 bp

Back to Gynostemma

Pi 0.0367
MarkerSeek score 76.90
Primer available yes
Label trnk-UUU-rps16

Feature Metadata

label_name
trnk-UUU-rps16
feature_type
igs
region
LSC
length_bp
1122
variable_sites
126
pi
0.0367
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.98
markerseek_score
76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnk-UUU-rps16_p1 CCGCACTTAAAAGCCGAGTA ACGGAACTTCACCTTAATCA 50.0 40.0 58.3 54.3 1087-1229 bp 1.0 60.1
trnk-UUU-rps16_p2 CCGCACTTAAAAGCCGAGTA ACGGAACTTCACCTTAATCAA 50.0 38.1 58.3 55.2 1087-1229 bp 1.0 59.0
trnk-UUU-rps16_p3 GTGTAAAAGATGCTAGCCGC ACGGAACTTCACCTTAATCA 50.0 40.0 57.3 54.3 1103-1245 bp 1.0 56.5
trnk-UUU-rps16_p4 CGCACTTAAAAGCCGAGTAC ACGGAACTTCACCTTAATCA 50.0 40.0 57.2 54.3 1086-1228 bp 1.0 56.1
trnk-UUU-rps16_p5 CCGCACTTAAAAGCCGAGTA GTTTTTACGGAACTTCACCT 50.0 40.0 58.3 53.8 1093-1235 bp 1.0 55.9