Hotspot

rbcL

LSC · 55071-56498 · 1428 bp

Back to Gynochthodes

Pi 0.0014

MarkerSeek score 59.60

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1428
variable_sites: 3
pi: 0.0014
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	TCATGAGTTGTAGGGAGGGA	AGTAAAAGATTGGGCCGAGT	50.0	45.0	57.1	56.8	1505 bp	1.0	63.2
rbcL_p2	TTCATGAGTTGTAGGGAGGGA	AGTAAAAGATTGGGCCGAGT	47.6	45.0	57.8	56.8	1506 bp	1.0	61.2
rbcL_p3	TCATGAGTTGTAGGGAGGGA	AGTAAAAGATTGGGCCGAGTT	50.0	42.9	57.1	57.6	1505 bp	1.0	61.2
rbcL_p4	TCATGAGTTGTAGGGAGGGAT	AGTAAAAGATTGGGCCGAGT	47.6	45.0	57.6	56.8	1505 bp	1.0	59.4
rbcL_p5	TTCATGAGTTGTAGGGAGGGA	AGTAAAAGATTGGGCCGAGTT	47.6	42.9	57.8	57.6	1506 bp	1.0	59.1