Hotspot

ccsA

SSC · 113332-114303 · 972 bp

Back to Gynochthodes

Pi 0.000686

MarkerSeek score 63.40

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 972
variable_sites: 1
pi: 0.000686
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	CGGCATGCCACCTTCTAAAA	CGGTTGGATTCGGAAGATCC	50.0	55.0	58.8	58.4	1077-1115 bp	1.0	73.1
ccsA_p2	CGGCATGCCACCTTCTAAAA	TCGGTTGGATTCGGAAGATC	50.0	50.0	58.8	57.4	1078-1116 bp	1.0	71.7
ccsA_p3	CGGCATGCCACCTTCTAAAA	CTCGGTTGGATTCGGAAGAT	50.0	50.0	58.8	57.4	1079-1117 bp	1.0	71.6
ccsA_p4	CGGCATGCCACCTTCTAAAA	CACTCGGTTGGATTCGGAAG	50.0	55.0	58.8	58.6	1081-1119 bp	1.0	71.3
ccsA_p5	CGGCATGCCACCTTCTAAA	CGGTTGGATTCGGAAGATCC	52.6	55.0	58.1	58.4	1077-1115 bp	1.0	70.7