Hotspot

rpl32-trnL-UAG

SSC · 112570-113143 · 574 bp

Back to Gynochthodes

Pi 0.00813

MarkerSeek score 64.00

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 574
variable_sites: 6
pi: 0.00813
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GTGGCTATTGGACAGCGTTA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	704-716 bp	1.0	80.0
rpl32-trnL-UAG_p2	GTGGCTATTGGACAGCGTTA	AGCAGCGTGTCTACCAATTT	50.0	45.0	58.0	57.8	697-709 bp	1.0	79.5
rpl32-trnL-UAG_p3	GTGGCTATTGGACAGCGTTA	CCTAAGAGCAGCGTGTCTAC	50.0	55.0	58.0	57.8	703-715 bp	1.0	79.4
rpl32-trnL-UAG_p4	GTGGCTATTGGACAGCGTTA	CTAAGAGCAGCGTGTCTACC	50.0	55.0	58.0	57.8	702-714 bp	1.0	79.4
rpl32-trnL-UAG_p5	GTGGCTATTGGACAGCGTTA	GAGCAGCGTGTCTACCAATT	50.0	50.0	58.0	58.3	698-710 bp	1.0	78.8