Pi
0.0078
MarkerSeek score
63.70
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | GCGATGCTCTAACCTCTGAG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.9 | 58.0 | 819-831 bp | 1.0 | 79.8 |
| trnT-UGU-trnL-UAA_p2 | GCGATGCTCTAACCTCTGAG | GTTTCCATACCAAGGCCCAA | 55.0 | 50.0 | 57.9 | 58.1 | 861-873 bp | 1.0 | 79.2 |
| trnT-UGU-trnL-UAA_p3 | GCGATGCTCTAACCTCTGAG | TAGCGTCTACCAATTTCGCC | 55.0 | 50.0 | 57.9 | 58.1 | 827-839 bp | 1.0 | 79.2 |
| trnT-UGU-trnL-UAA_p4 | GGAATCGAACCGATGACCAT | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.8 | 58.0 | 851-863 bp | 1.0 | 78.9 |
| trnT-UGU-trnL-UAA_p5 | GGAATCGAACCGATGACCAT | GTTTCCATACCAAGGCCCAA | 50.0 | 50.0 | 57.8 | 58.1 | 893-905 bp | 1.0 | 78.3 |