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petN-psbM

LSC · 29006-29767 · 762 bp

Back to Gynochthodes

Pi 0.0105

MarkerSeek score 66.50

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 762
variable_sites: 12
pi: 0.0105
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	TCATTCTAGTTCCTACGGCCT	50.0	47.6	58.2	58.0	909-968 bp	1.0	72.2
petN-psbM_p2	GGGGACACAATTCACATGGA	TCATTCTAGTTCCTACGGCCT	50.0	47.6	57.8	58.0	931-990 bp	1.0	71.7
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	TCTAGTTCCTACGGCCTTTCT	50.0	47.6	58.2	57.9	905-964 bp	1.0	71.7
petN-psbM_p4	AGGGGACACAATTCACATGG	TCATTCTAGTTCCTACGGCCT	50.0	47.6	57.8	58.0	932-991 bp	1.0	71.6
petN-psbM_p5	GGCTGCTTTAATGGTCGTCT	TCATTCTAGTTCCTACGGCCT	50.0	47.6	58.3	58.0	893-952 bp	1.0	71.4