Hotspot

rps16-trnQ-UUG

LSC · 6187-7414 · 1228 bp

Back to Gynochthodes

Pi 0.00977

MarkerSeek score 62.10

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1228
variable_sites: 17
pi: 0.00977
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1364-1375 bp	1.0	80.1
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	AGCAATCTAAATGGGGCGTG	45.0	50.0	58.1	58.6	1423-1434 bp	1.0	75.8
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	45.0	50.0	58.1	57.3	1368-1379 bp	1.0	75.4
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	TAAGGCAACGGGTTTTGGTC	45.0	50.0	58.1	58.7	1394-1405 bp	1.0	75.3
rps16-trnQ-UUG_p5	CGTTGCTTTCTACCACATCG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.2	58.1	1356-1367 bp	1.0	75.0