Hotspot

petD

LSC · 81800-82692 · 893 bp

Back to Gymnotheca

Pi 0.00336

MarkerSeek score 60.80

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 893
variable_sites: 3
pi: 0.00336
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	TTCCTGTGCATCAATTCCGA	AGGGAGGATTCGCTTTGAAG	45.0	50.0	57.5	57.6	989-990 bp	1.0	73.1
petD_p2	TCCTGTGCATCAATTCCGAT	AGGGAGGATTCGCTTTGAAG	45.0	50.0	57.3	57.6	988-989 bp	1.0	71.3
petD_p3	TTCCTGTGCATCAATTCCGA	GCACGATCCGTAGATTCGTA	45.0	50.0	57.5	57.3	1042-1043 bp	1.0	71.0
petD_p4	TCCTGTGCATCAATTCCGAT	GCACGATCCGTAGATTCGTA	45.0	50.0	57.3	57.3	1041-1042 bp	1.0	69.2
petD_p5	TTCCTGTGCATCAATTCCGAT	AGGGAGGATTCGCTTTGAAG	42.9	50.0	58.0	57.6	989-990 bp	1.0	69.0