Hotspot

ndhA

SSC · 126541-128749 · 2209 bp

Back to Gymnotheca

Pi 0.00226

MarkerSeek score 58.60

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2209
variable_sites: 5
pi: 0.00226
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGAACCCAGTCATCATAGGGA	ATTGGCCGATATCATGACGA	47.6	45.0	57.9	56.9	2382-2384 bp	1.0	62.1
ndhA_p2	TGAACCCAGTCATCATAGGGA	TTGGCCGATATCATGACGAT	47.6	45.0	57.9	56.9	2381-2383 bp	1.0	62.1
ndhA_p3	ACCCAGTCATCATAGGGAACA	ATTGGCCGATATCATGACGA	47.6	45.0	58.2	56.9	2379-2381 bp	1.0	61.9
ndhA_p4	ACCCAGTCATCATAGGGAACA	TTGGCCGATATCATGACGAT	47.6	45.0	58.2	56.9	2378-2380 bp	1.0	61.9
ndhA_p5	TGAACCCAGTCATCATAGGGA	AATTGGCCGATATCATGACGA	47.6	42.9	57.9	57.6	2383-2385 bp	1.0	59.9