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psbT-psbN

LSC · 79188-79250 · 63 bp

Back to Gymnotheca

Pi 0.0159

MarkerSeek score 61.60

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 63
variable_sites: 1
pi: 0.0159
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	ACCACCTAGGATTTCGACGA	ATCCATTCGAAGAACACGGG	50.0	50.0	58.2	58.0	123 bp	1.0	79.2
psbT-psbN_p2	ACCACCTAGGATTTCGACGA	TTCGAAGAACACGGGGACTA	50.0	50.0	58.2	58.4	118 bp	1.0	76.4
psbT-psbN_p3	AACCACCTAGGATTTCGACG	ATCCATTCGAAGAACACGGG	50.0	50.0	57.3	58.0	124 bp	1.0	75.1
psbT-psbN_p4	ACCACCTAGGATTTCGACGA	GGCAACCCTCTCAACAACTA	50.0	50.0	58.2	57.4	147 bp	1.0	74.8
psbT-psbN_p5	CCACCTAGGATTTCGACGAA	ATCCATTCGAAGAACACGGG	50.0	50.0	57.0	58.0	122 bp	1.0	72.8