Hotspot

accD-psaI

LSC · 62670-63382 · 713 bp

Back to Gymnotheca

Pi 0.00143

MarkerSeek score 61.20

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 713
variable_sites: 1
pi: 0.00143
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AGCTACACGGTTTCTTTCCT	TTGCAATTGCCGGGAATACT	45.0	45.0	56.8	58.2	813-824 bp	1.0	70.2
accD-psaI_p2	CAGCTACACGGTTTCTTTCCT	TTGCAATTGCCGGGAATACT	47.6	45.0	58.2	58.2	814-825 bp	1.0	70.2
accD-psaI_p3	TCAGCTACACGGTTTCTTTCC	TTGCAATTGCCGGGAATACT	47.6	45.0	58.2	58.2	815-826 bp	1.0	70.1
accD-psaI_p4	CAGCTACACGGTTTCTTTCC	TTGCAATTGCCGGGAATACT	50.0	45.0	56.7	58.2	814-825 bp	1.0	70.0
accD-psaI_p5	AGCTACACGGTTTCTTTCCT	CCGGGAATACTAGGCCTACT	45.0	55.0	56.8	57.7	804-815 bp	1.0	69.0