Hotspot

trnF-GAA-ndhJ

LSC · 51255-51953 · 699 bp

Back to Gymnotheca

Pi 0.00143

MarkerSeek score 59.60

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 699
variable_sites: 1
pi: 0.00143
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	TGAAAGTTGGATAGGCTGGC	55.0	50.0	58.0	57.9	845 bp	1.0	79.0
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	809 bp	1.0	78.7
trnF-GAA-ndhJ_p3	GGGATAGCTCAGCTGGTAGA	ATAGGCTGGCCCTTACGTAA	55.0	50.0	58.0	58.2	835 bp	1.0	78.5
trnF-GAA-ndhJ_p4	GGGATAGCTCAGCTGGTAGA	GAGAATCATCCGCGTCTGAA	55.0	50.0	58.0	57.8	880 bp	1.0	78.4
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	ATAGGCTGGCCCTTACGTAA	50.0	50.0	58.1	58.2	799 bp	1.0	78.1