Hotspot

petN-psbM

LSC · 30120-31292 · 1173 bp

Back to Gymnotheca

Pi 0.00426

MarkerSeek score 61.20

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1173
variable_sites: 5
pi: 0.00426
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	ATTGCTACTGCGCTGTTCAT	50.0	45.0	58.2	58.3	1337 bp	1.0	77.0
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	TATTGCTACTGCGCTGTTCA	50.0	45.0	58.2	57.6	1338 bp	1.0	76.1
petN-psbM_p3	ATGGGGAAGAAGTGGACTCT	ATTGCTACTGCGCTGTTCAT	50.0	45.0	57.6	58.3	1276 bp	1.0	75.4
petN-psbM_p4	TAGTAAGTCTCGCTTGGGCT	TTGCTACTGCGCTGTTCATT	50.0	45.0	58.2	58.5	1336 bp	1.0	75.3
petN-psbM_p5	ATGGGGAAGAAGTGGACTCT	TATTGCTACTGCGCTGTTCA	50.0	45.0	57.6	57.6	1277 bp	1.0	74.5