Hotspot

rpoB-trnC-GCA

LSC · 28243-29521 · 1279 bp

Back to Gymnotheca

Pi 0.00392

MarkerSeek score 61.20

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1279
variable_sites: 5
pi: 0.00392
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TGAAGTTCTTCGGTCAAGCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.1	58.1	1448 bp	1.0	78.8
rpoB-trnC-GCA_p2	GTCAAGCCCTGATCAACGAA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.2	58.1	1436 bp	1.0	78.2
rpoB-trnC-GCA_p3	TCAAGCCCTGATCAACGAAC	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.2	58.1	1435 bp	1.0	78.2
rpoB-trnC-GCA_p4	GCCCTGATCAACGAACCTAC	CCGGATTTGAACTGGGGAAA	55.0	50.0	58.3	58.1	1431 bp	1.0	77.0
rpoB-trnC-GCA_p5	TGAAGTTCTTCGGTCAAGCC	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.1	58.4	1430 bp	1.0	76.6