Hotspot

rps16-trnQ-UUG

LSC · 6173-7654 · 1482 bp

Back to Gymnotheca

Pi 0.00202
MarkerSeek score 59.90
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1482
variable_sites
3
pi
0.00202
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 TATGTCCTTCAAGTCGCACG TGGTCCTGTTATTCGGAGGT 50.0 50.0 58.0 58.1 1581-1582 bp 1.0 79.7
rps16-trnQ-UUG_p2 TATGTCCTTCAAGTCGCACG GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1566-1567 bp 1.0 79.5
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA TGGTCCTGTTATTCGGAGGT 45.0 50.0 58.1 58.1 1571-1572 bp 1.0 78.6
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1556-1557 bp 1.0 78.4
rps16-trnQ-UUG_p5 TATGTCCTTCAAGTCGCACG CCGGGTTTTGGTCCTGTTAT 50.0 50.0 58.0 57.8 1589-1590 bp 1.0 78.4