Hotspot

rpl16

LSC · 83548-85063 · 1516 bp

Back to Gymnosporia

Pi 0.00462

MarkerSeek score 47.00

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1516
variable_sites: 7
pi: 0.00462
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TGTCAGCAAGAGTGTCCCTA	CAAGTACAAATTGCAGGGCG	50.0	50.0	58.0	58.0	2572-2575 bp	1.0	80.2
rpl16_p2	TGTCAGCAAGAGTGTCCCTA	GCAGAATTTCTAGCCGGACA	50.0	50.0	58.0	58.0	2671-2674 bp	1.0	80.2
rpl16_p3	TGTCAGCAAGAGTGTCCCTA	TGCAGAATTTCTAGCCGGAC	50.0	50.0	58.0	58.0	2672-2675 bp	1.0	80.2
rpl16_p4	ACAGCCAAAAGGCGAAGTAA	CAAGTACAAATTGCAGGGCG	45.0	50.0	58.0	58.0	1984-1986 bp	1.0	80.2
rpl16_p5	ACAGCCAAAAGGCGAAGTAA	GCAGAATTTCTAGCCGGACA	45.0	50.0	58.0	58.0	2083-2085 bp	1.0	80.1