Hotspot

trnI-GAU-rrn16

IRa · 139573-139867 · 295 bp

Back to Gymnosporia

Pi 0.0305
MarkerSeek score 70.00
Primer available yes
Label trnI-GAU-rrn16

Feature Metadata

label_name
trnI-GAU-rrn16
feature_type
igs
region
IRa
length_bp
295
variable_sites
9
pi
0.0305
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnI-GAU-rrn16_p1 CTATCCATGTGGCTGAGAGC CAAGGTAGCCGTACTGGAAG 55.0 55.0 57.8 57.7 421 bp 1.0 78.0
trnI-GAU-rrn16_p2 GGCACAACGACGCAATTATC CAAGGTAGCCGTACTGGAAG 50.0 55.0 58.2 57.7 389 bp 1.0 78.0
trnI-GAU-rrn16_p3 CGCTGATGAGCACATTGAAC CAAGGTAGCCGTACTGGAAG 50.0 55.0 57.8 57.7 440 bp 1.0 77.9
trnI-GAU-rrn16_p4 CTATCCATGTGGCTGAGAGC AGTGACTGGAGTGAAGTCGT 55.0 50.0 57.8 58.3 443 bp 1.0 77.8
trnI-GAU-rrn16_p5 CGCTGATGAGCACATTGAAC AGTGACTGGAGTGAAGTCGT 50.0 50.0 57.8 58.3 462 bp 1.0 77.8